Students in Mrs. Shulamith Biderman and Mrs. RuthFried’s Forensics and AP Biology classes at Central attended DNALC West, where they used chip analysis and bacterial transformation to conduct experiments in human DNA fingerprinting. The forensic DNA profiling lab examined a highly variable tandem repeat polymorphism, like the ones used by the FBI for DNA fingerprinting. Coupling chip analysis with a new, fast amplification protocol allowed students to isolate, amplify, and analyze their own DNA.
The bacterial transformation experiment illustrated the direct link between an organism’s genotype and phenotype. Students were excited by the opportunity to apply the lab techniques they learned in their science classes in a real-world context.
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